what special treatment you will give while cleaning infected glassware
please it's very urgent
Answers
Answer:
aqua regia by pouring it into a second container that will be treated and disposed of and we use simple acid and base to clean the glassware.
Explanation:
Answer:
Cleaning Laboratory Glassware
Wash labware as quickly as possible after use. If labware is not cleaned immediately, it may become impossible to remove any residue.
If a thorough cleaning is not possible immediately, put glassware to soak in water.
Most new glassware items are slightly alkaline in reaction. For precision chemical testing, new glassware should be soaked for several hours in acid water (a 1% solution of hydrochloric or nitric acid) before proceeding with a regular washing proceedure.
Brushes with wooden or plastic handles are recommended as they will not scratch or otherwise abrade the glassware's surface.
Glassware Cleaners
When washing, soap, detergent, or cleaning powder (with or without an abrasive) may be used. Cleaners for glassware include Alconox®, Dural®,M&H®, Lux®, Tide® and Fab®. The water should be hot. For glassware that is exceptionally dirty, a cleaning powder with a mild abrasive action will give more satisfactory results. The abrasive should not scratch the glass. During the washing, all parts of the glassware should be thoroughly scrubbed with a brush. This means that a full set of brushes must be at hand, including brushes to fit large and small test tubes, burets, funnels, graduates, and various sizes of flasks and bottles. Motor driven revolving brushes are valuable when a large number of tubes or bottles are processed. Do not use cleaning brushes that are so worn that the spine hits the glass. Serious scratches may result. Scratched glass is more prone to break during experiments. Any mark in the uniform surface of glassware is a potential breaking point, especially when the piece is heated. Do not allow acid to come into contact with a piece of glassware before the detergent (or soap) is thoroughly removed. If this happens, a film of grease may be formed.
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Safe Use of Chromic Acid
If glassware becomes unduly clouded or dirty or contains coagulated organic matter, it must be cleansed with chromic acid cleaning solution.1 The dichromate should be handled with extreme care because it is a powerful corrosive and carcinogen.
When chromic acid solution is used the item may be rinsed with the cleaning solution or it may be filled and allowed to stand. The length of time it is allowed to stand depends on the amount of contamination on the glassware. Relatively clean glassware may require only a few minutes of exposure; if debris is present, such as blood clots, it may be necessary to let the glassware stand all night. Due to the intense corrosive action of the chromic acid solution, it is good practice to place the stock bottle, as well as the glassware being treated, in flat glass pans, pans made from lead or coated with lead, or plastic polymer pans determined to be compatible with the concentration of chromic acid you are using. Extra care must be taken to be sure chromic acid solution is disposed of properly.
Special types of precipitates may require removal with nitric acid, aqua regia, or fuming sulfuric acid. These are very corrosive substances and should be used only when required.
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Removing Grease
Grease is best removed by boiling in a weak solution of sodium carbonate. Acetone or any other fat solvent may be used. Strong alkalis should not be used. Silicone grease is most easily removed by soaking the stopcock plug or barrel for 2 hours in warm decahydronaphthalene.
Drain and rinse with acetone or use fuming sulfuric acid for 30 minutes. Be sure to rinse off all of the cleaning agents.
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Rinsing
It is imperative that all soap, detergents, and other cleaning fluids be removed from glassware before use. This is especially important with the detergents, slight traces of which will interfere with serologic and cultural reactions.
After cleaning, rinse the glassware with running tap water. When test tubes, graduates, flasks, and similar containers are rinsed with tap water, allow the water to run into and over them for a short time, then partly fill each piece with water, thoroughly shake and empty at least six times. Pipets and burets are best rinsed by attaching a piece of rubber tubing to the faucet and then attaching the delivery end of the pipets or burets to a hose, allowing the water to run through them. If the tap water is very hard, it is best to run it through a deionizer before using.
Rinse the glassware in a large bath of distilled water. Rinse with distilled water. To conserve distilled water, use a five gallon bottle as a reservoir. Store it on a shelf near your clean-up area. Attach a siphon to it and use it for replenishing the reservoir with used distilled water. For sensitive microbiologic assays, meticulous cleaning must be followed by rinsing 12 times in distilled water.