Question

what strategy will be used to identify and clone this gene?​

Answer 1

Answer:

There are two types of gene cloning: in vivo, which involves the use of restriction enzymes and ligases using vectors and cloning the fragments into host cells (as can be seen in the image above). The other type is in vitro which is using the polymerase chain reaction (PCR) method to create copies of fragments of DNA.

Answer 2

Answer:

One way of detecting a specific cloned gene is by detecting its protein product expressed in the bacterial cell. Therefore, in these cases, it is necessary to be able to express the gene in bacteria; that is, to transcribe it and translate the mRNA into protein.