What the procedure and precautions you will follow to observe Onion peel cells in the laboratory.
Answers
Question:
What procedure and precautions will u follow to observe Onion Pell Cells In the Laboratory?
Precautions:
Use a brush to transfer the peel from one apparatus to another.
Staining of peel should neither be too dark, nor too light.
Extra glycerine stain should be removed using blotting paper.
Procedure:
Pour some distilled water into a watch glass.
Peel off a leaf from half a piece of onion and using the forceps, pull out a piece of transparent onion peel (epidermis) from the leaf.
Put the epidermis in the watch glass containing distilled water.
Take a few drops of safranin solution in a dropper and transfer this into another watch glass.
Using a brush, transfer the peel into the watch glass containing the safranin solution.
Let this remain in the Safranin solution for 30 seconds, so that the peel is stained.
Take the peel from the Safranin solution using the brush and place it in the watch glass containing the distilled water.
Take a few drops of glycerine in a dropper and pour 2-3 drops at the center of a dry glass slide.
Using the brush, place the peel onto the slide containing glycerine.
Take a cover slip and place it gently on the peel with the aid of a needle.
Remove the extra glycerine using a piece of blotting paper.
Place this glass side on the stage of the compound microscope and view it.
Answer:
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