what the tests of lipid?
Answers
Explanation:
Ethanol Emulsion Test is a food test which determines the presence of a broad group of naturally occurring compounds known as lipids. Lipids consist of fats and oils. Other lipid tests include the Grease Spot Test and the Sudan Stain Test. ... The Ethanol Emulsion Test is the most common test amongst the three.
heya mate...
three main tests for lipids:-
1. General Test for Lipid
2. Solubility Test for Lipid
3. Emulsification Test for Lipid.
General Test for Lipid:
1. Sudan III test Procedure:
Take 0.5 ml ether or chloroform in a test tube and add 0.5 ml sample—drop by drop till the sample is fully dissolves. Add one drop of Sudan III reagent.
2. Acrolein test:
Procedure:
Take 0.5gm powdered sodium bi-sulphate (NaHSO4) or potassium bi-sulphate (KHSO4) in a clean dry test tube, add 3 to 4 drops of sample. Mix thoroughly and heat.
Observation:
An irritating smell of acrolein is felt.
3 solubility test
Take five test tubes marking A, B, C, D, E. Put 5 ml—water, absolute alcohol, ether, chloroform and benzene one in each test tube. Add 3 to 4 drops of sample in each test tube, shake thoroughly, allow to stand.
Observation:
(1) In test tube A drops of oils are seen floating on the surface of water,
(2) In test tube B oil drops settle at the bottom of alcohol,