Question

What was used by Ruben to confirm that the source of oxygen evolved in photosynthesis is water​

Answer 1

Explanation:

Ruben's experiments using 'heavy water', H218O, to yield 18O2 gas had shown that the oxygen gas produced in photosynthesis comes from water.

• By comparison, the second group of plants was given radioactively-labeled carbon dioxide and unlabeled water. Results showed that the oxygen released from the first group of plants was radioactive, confirming that water is the source of the oxygen produced by photosynthesis.

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Answer 2

Ruben's experiments using 'heavy water', H218O, to yield 18O2 gas had shown that the oxygen gas produced in photosynthesis comes from water. Ruben fully realized the potential use of this isotope and was committed to working to elucidate the mechanism of photosynthetic carbon fixation using carbon-14.