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If one of the trigonometric ratios of an acute angle is known, the remaining trigonometric ratios of the angle can be easily determined
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Because cells typically have negatively charged cell walls, the positive chromophores in basic dyes tend to stick to the cell walls, making them positive stains. Thus, commonly used basic dyes such as basic fuchsin, crystal violet, malachite green, methylene blue, and safranin typically serve as positive stains.
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