Question

What will happen if a groth media is inculated with lysogen?

Answer 1

$\underline\bold{\huge{Answer !!}}$

1. Grow a colony of recombinant λ-phage lysogen in 50 mL of LB broth with the appropriate antibiotic overnight at 30°C.

2.Use 10 mL of this starter culture to inoculate 500 mL of LB the following morning. When the flask reaches an OD600 ~ 0.6, rapidly raise the temperature to 42°C by swirling in a preheated water bath.

3.Once the temperature reaches 42°C, induce lytic growth by transferring the culture to a 45°C shaking incubator for 15 min.

4.After 15 min, lower the incubator temperature to 37°C and continue shaking for 2 h.