what would be the out come if the iodine was not used in gram staining
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During the gram staining process, the gram-positive bacteria appear violet because it has a thick peptidoglycan layer. ... The iodine acts as mordant and it does not use as a stain. It helps to fix the crystal violet inside the peptidoglycan layer. If iodine is not added, it would appear purple rather than pink.
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