Question

When and who discovered lactophenol cotton blue stain?

Answer 1

The lactophenol cotton blue (LPCB) wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare. The preparation has three components:

Phenol:   kills any live organisms;

Lactic acid : It  preserves fungal structures, and

Cotton blue : It stains the chitin in the fungal cell walls.