Chemistry, asked by niksrcm4363, 1 year ago

When can you equilibrate ni-nta column with lysis buffer?

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Answered by gauravarduino

Answer:

exposed, it will facilitate the binding to Ni columns. ... We describe here two protein purification procedures. ... 6 Equilibrate 50µl Nil beads with equilibration buffer (see Equilibration of Ni-NTA agarose).

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