Question

When chromatin is treated with non specific nucleases what is the length of the resultingpieces of DNA

Answer 1

Answer:

v v v hm

Explanation:

/ j j hkh  

Answer 2

Non-specific nucleases generally cleave the chromatin threads into random fragments of various lengths and sizes.

• Non- specific nucleases belong to a classification of nucleases that can degrade both single and double-stranded DNA and RNA without any target specification.
• They do not show any sequence specificity during degradation and randomly breaks down the genetic material into several pieces of strands differing in both size and length.
• They can be extracted from various sources such as bacteria, fungi, viruses etc. Example: Non-specific nuclease from Yersinia enterocolitica subspecies palearctica having high catalytic activity and good thermostability.
• These nucleases are also exploited for studying nucleic acid metabolism, cell processes such as DNA repair, RNA splicing, transposition, topoisomerisation, nucleoside deletion etc.