Question

When foreign DNA is inserted into any vector, it results in inactivation of any marker gene.this is used for the selection of

Answer 1

Hey Dear,

● Answer -

When foreign DNA is inserted into any vector, it results in inactivation of any marker gene. This is used for the selection of recombinant colonies.

● Explanation -

• Insertional inactivation is a technique of r-DNA technology where a insertion of DNA-fragment into restriction site inactivates the gene.
• This technique is used for selection of recombinant colonies.
• This method can be used for inactivating genes for antibiotic resistance.

Hope this helps you.

Answer 2

Answer:

Plasmids and bacteriophages can imitate inside bacterial cells free of the control of chromosomal DNA. Bacteriophages on account of their high number per cell, have extremely high duplicate quantities of their genome inside the bacterial cells. A few plasmids may have just a couple of duplicates for every cell while others may have  15-100 duplicates for every cell. Their numbers can go considerably higher.

In the event that we are capable to interface an outsider bit of DNA with bacteriophage or plasmid DNA, we can duplicate its numbers equivalent to the duplicate number of the plasmid or bacteriophage. Vectors utilized at present, are built in such a way that they help simple connecting of remote DNA and determination of recombinants from non-recombinants.