When is reverse phase hplc used instead of normal phaae?
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It is typical for normal-phase chromatography on silica that the mobile phase is 100% organic; no water is used. The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar [hydrophobic] stationary phase.
It is great when a compound is too hydrophobic or hydrophilic for reverse-phase HPLC. It can also be used for isomer separation, if the sample injection solvent is non-polar, or if recovery in non-polar solvents is desirable. In normal phase chromatography, the stationary phase is polar, usually using silica.
It is great when a compound is too hydrophobic or hydrophilic for reverse-phase HPLC. It can also be used for isomer separation, if the sample injection solvent is non-polar, or if recovery in non-polar solvents is desirable. In normal phase chromatography, the stationary phase is polar, usually using silica.
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Explanation:
Reverse Phase HPLC
This technique can be used to separate, identify and or quantitate components in mixtures of soluble organic components based on their hydrophobicity. The stationary phase is nonpolar, like C18 bonded silica. The mobile phase is polar, usually being water and polar organic solvent.
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