Question

When setting up Sanger sequencing
reaction, each reaction should include
template DNA, nucleotides,
dideoxynucleotides, buffer, DNA
polymerase and
Forward AND reverse primers
Forward OR reverse primers
Forward AND reverse probes
Forward OR reverse probes​

Answer 1

Answer:

Primers are short sequences of single stranded DNA that mark both ends of the target sequence. ... The forward primer attaches to the start codon of the template DNA (the anti-sense strand), while the reverse primer attaches to the stop codon of the complementary strand of DNA (the sense strand).

Explanation:

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Answer 2

Answer:

Forward AND reverse primers