Question

When the gene encoding a certain cytoskeleton protein is deleted, the resulting mutant cells round up and do not form their normal appendages. These mutants can be rescued when a gene encoding an N-terminal green fluorescent protein (GFP) fusion of the protein is expressed, but not when a gene encoding a C-terminal GFP fusion is expressed. Which fusion protein is appropriate to use in studying cellular localization and activity

Answer 1

Answer:

Three of the most important uses of fusion proteins are: as aids in the purification of cloned genes, as reporters of expression level, and as histochemical tags to enable visualization of the location of proteins in a cell, tissue, or organism.

Explanation:

Answer 2

Answer:

N-terminal green fluorescent protein (N-terminal GFP)

Explanation:

Since the mutant protein is rescued when tagged with N-terminal GFP, it will help in identifying the cellular localization and activity of the protein. The rescued protein is visualized through fluorescence microscopy and its localization can be visualized in real-time with the help of GFP.