Question

When using a compound light microscope, why is it important to first focus in the lowest powered objective

Answer 1

When you change from low power to high power on a microscope, the high-power objective lens moves directly over the specimen, and the low-power objective lens rotates away from the specimen. This change alters the magnification of a specimen, the light intensity, area of the field of view, depth of field, working distance and resolution. The image should remain in focus if the lenses are of high quality.

Answer 2

Answer:

When using a light microscope it's important to start with the low power objective lens as the field of view will be wider, increasing the number of cells you are able to see. This makes it easier to find what you're looking for.