where are ribosomes prepared
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Ribosomes, the large protein–RNA complexes involved in translation of nucleotide sequences into proteins, are obtained from cells broken open by grinding, shear forces or enzymatically. They are purified from the cell lysate by centrifugation to remove cell debris, followed by sucrose density gradient centrifugation.
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Ribosome biogenesis is the process of making ribosomes. In prokaryotes, this process takes place in the cytoplasm with the transcription of many ribosome gene operons. In eukaryotes, it takes place both in the cytoplasm and in the nucleolus.
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