which are the various processes in tissue culture
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Tissue culture is a process that involves exposing plant tissue to a specific regimen of nutrients, hormones, and light under sterile, in vitro conditions to produce many new plants, each a clone of the original mother plant, over a very short period of time. AgriForest’s tissue culture plants are characterised by disease free growth, a more fibrous, healthier root system, a bushier branching habit, and a higher survival rate.
There are three main steps to the tissue culture process. Following these three stages, the plants are then moved from the laboratory to the greenhouses for acclimatisation and further development.
STAGE I is the initiation phase. It concerns the establishment of plant tissue in vitro by sterilizing the material and initiating it into culture.
STAGE II is the multiplication phase. At this stage, the in vitro plant material is re-divided and placed in a medium with plant growth regulators that induce the proliferation of multiple shoots. This process is repeated many times until the number of plants desired is reached.
STAGE III is the root formation phase. It involves the introduction of hormones to induce rooting and the formation of complete plantlets.
There are three main steps to the tissue culture process. Following these three stages, the plants are then moved from the laboratory to the greenhouses for acclimatisation and further development.
STAGE I is the initiation phase. It concerns the establishment of plant tissue in vitro by sterilizing the material and initiating it into culture.
STAGE II is the multiplication phase. At this stage, the in vitro plant material is re-divided and placed in a medium with plant growth regulators that induce the proliferation of multiple shoots. This process is repeated many times until the number of plants desired is reached.
STAGE III is the root formation phase. It involves the introduction of hormones to induce rooting and the formation of complete plantlets.
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