Question

Which bacterial strain is good for plasmid isolation?

Answer 1

DH10B

In general, DH5alpha and DH10B are good strains to try for this purpose since they lack restriction/modification systems that can prevent plasmid propagation, lack endonuclease activity that can lead to cleavage of certain plasmid sequences, and carry mutations that enable the uptake of large plasmids.

Answer 2

In general, DH5alpha and DH10B are good strains to try for this purpose since they lack restriction/modification systems that can prevent plasmid propagation, lack endonuclease activity that can lead to cleavage of certain plasmid sequences, and carry mutations that enable the uptake of large plasmids.