Which buffer imparts positive charge to protiens in sds page?
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Answer:
Most SDS PAGE sample buffers contain the following: SDS (sodium dodecyl sulphate, also called lauryl sulphate), b-mercaptoethanol (BME), bromophenol blue, glycerol, and Tris-glycine at pH 6.8. BME is added to prevent oxidation of cysteines and to break up disulfide bonds.
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Explanation:
sodium dodicyl sulphate...
sodium di bromol
glycarol..
tri glysarol..
bromophenol blue
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