Question

Which buffer solution and indicators are used in EDTA method?

Answer 1

Answer:

EBT is used as indicator and for buffer NH4Cl and ammonia.

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Yes this is me if you are here for assignment 2

Answer 2

Ammonia buffer solution and Eriochrome Black T indicators are used in the EDTA method.

Explanation:

• In EDTA titration a buffer solution is used because it resists the change in pH.
• All the reactions which take place between metal ions and EDTA are pH-dependent.
• EDTA is a chelating agent which binds divalent metal ions such as Calcium, Magnesium.
• It can be used the stop the degradation of DNA and RNA.
• naIt is used to inactivate the nucleases which require metal ions.
• It can also be used to inactivate metal ions requiring enzymes.
• Ammonia buffer ($NH4Cl +NH4OH$) and EBT Eriochrome black T indicators are the buffer and indicator.