which chemical test should performed to differentiate between glycine and arginine
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Answer:The Van Slyke determination tests for specific amino acids
Explanation:The Van Slyke determination is a chemical test for the determination of amino acids containing a primary amine group. It is named after the biochemist Donald Dexter Van Slyke (1883-1971).[1]
One of Van Slyke's first professional achievements was the quantification of amino acids by the Van Slyke determination reaction.[2] To quantify aliphatic amino acids, the sample is diluted in glycerol and then treated with a solution of sodium nitrite, water and acetic acid. The resulting diazotisation reaction produces nitrogen gas which can be observed qualitatively or measured quantitatively.[3]
Van Slyke Reaction: R-NH2 + HONO → ROH + N2 + H2O [4]
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Glycine and Arginine Group
Explanation:
- The primary concern that makes amino acids not the same as each other is the R-group of every individual amino acid
- Glycine is a non-polar amino acid and arginine is a polar amino acid (The arginine containing the electronegative component of nitrogen)
- The main difference between these two amino acids is that glycine will have a R-group of a single hydrogen atom, where arginine's R-group will comprise of three CH2 units bound to one guanidino group
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