Biology, asked by shubhangirajput824, 1 month ago

Which colour developing agent is used for estimation of creatinine in urine by colorimeter?​

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Answered by Mister360
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Answer:

Question:-

Which colour developing agent is used for estimation of creatinine in urine by colorimeter?

Answer:-

  • Creatinine in serum or urine is determined by Jaffe's reaction where creatinine produces quantitatively an orange color with picric acid in alkaline medium.
  • After allowing an incubation time of 15 min at room temperature for color development the color is measured at 520 nm.
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