Which colour developing agent is used for estimation of creatinine in urine by colorimeter?
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Which colour developing agent is used for estimation of creatinine in urine by colorimeter?
Answer:-
- Creatinine in serum or urine is determined by Jaffe's reaction where creatinine produces quantitatively an orange color with picric acid in alkaline medium.
- After allowing an incubation time of 15 min at room temperature for color development the color is measured at 520 nm.
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