which colouring reagent used in your classroom to see Nucleus
Answers
Answer:
Safranin is a nuclear stain used as a counterstain and it can also be used to color collagen yellow. Nile blue used in living cells to stain nucleus blue.
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Answer:
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Answer
Hint: Nucleus of a cell contains nucleic acid associated with nucleoproteins. Thus, in order to observe a nucleus of a cell the stain used should impart color to the nucleic acid and/or the nucleoprotein. As nucleic acid is acidic and the nucleoprotein is basic in nature, both acidic and basic dyes can be used to stain a nucleus of a cell.
Hint: Nucleus of a cell contains nucleic acid associated with nucleoproteins. Thus, in order to observe a nucleus of a cell the stain used should impart color to the nucleic acid and/or the nucleoprotein. As nucleic acid is acidic and the nucleoprotein is basic in nature, both acidic and basic dyes can be used to stain a nucleus of a cell.Complete answer:
Hint: Nucleus of a cell contains nucleic acid associated with nucleoproteins. Thus, in order to observe a nucleus of a cell the stain used should impart color to the nucleic acid and/or the nucleoprotein. As nucleic acid is acidic and the nucleoprotein is basic in nature, both acidic and basic dyes can be used to stain a nucleus of a cell.Complete answer:Although there are various nuclear stains, Methylene blue is the most commonly used nuclear stain to observe the nucleus in the cheek cells. Methylene blue stain is preferred over other dyes due to its simple protocol, can be used without any mordant, and can be observed and distinguished even under a simple light microscope. Also, cheek cells are stained as fixed cells and methylene blue stains can stain fixed cells easily.
Hint: Nucleus of a cell contains nucleic acid associated with nucleoproteins. Thus, in order to observe a nucleus of a cell the stain used should impart color to the nucleic acid and/or the nucleoprotein. As nucleic acid is acidic and the nucleoprotein is basic in nature, both acidic and basic dyes can be used to stain a nucleus of a cell.Complete answer:Although there are various nuclear stains, Methylene blue is the most commonly used nuclear stain to observe the nucleus in the cheek cells. Methylene blue stain is preferred over other dyes due to its simple protocol, can be used without any mordant, and can be observed and distinguished even under a simple light microscope. Also, cheek cells are stained as fixed cells and methylene blue stains can stain fixed cells easily.Figure 1: Methylene blue-stained cheek cells under a microscope
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