which components are separated in chromatography?give examples
Answers
Answer:
Paper chromatography is an analytical method used to separate colored chemicals or substances.[1] It is primarily used as a teaching tool, having been replaced by other chromatography methods, such as thin-layer chromatography. A paper chromatography variant, two-dimensional chromatography involves using two solvents and rotating the paper 90° in between. This is useful for separating complex mixtures of compounds having similar polarity, for example, amino acids. The setup has three components. The mobile phase is a solution that travels up the stationary phase, due to capillary action. The mobile phase is generally mixture of non-polar organic solvent, while the stationary phase is polar inorganic solvent water. Here paper is used to support the stationary phase, water. Polar water molecules are held inside the void space of cellulose network of the host paper. Difference between TLC and paper chromatography is that stationary phase in TLC is a layer of adsorbent (usually silica gel, or aluminium oxide), and stationary phase in paper chromatography is water.
Paper chromatography
Chromatography tank.png
paper chromatography
Acronym
PC
Classification
Chromatography
Analytes
chromatography is a technique used for separation of the parts of a mixture of either gas or liquid solution
Other techniques
Related
Thin layer chromatography
Answer:
Ion exchange chromatography separates the components of a mixture based on their charge, in addition to or instead of their size. In essence, positively (cations) or negatively (anions) charged ions are separated using different stationary phases and different pH mobile ph