which concept forms the basis for colorimeter and spectrophotometer
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Introduction
A colorimeter is a light-sensitive device used for measuring the transmittance and absorbance of light passing through a liquid sample. The device measures the intensity or concentration of the color that develops upon introducing a specific reagent into a solution.
There are two types of colorimeters — color densitometers, which measure the density of primary colors, and color photometers, which measure the color reflection and transmission.
Design of Colorimeter
The three main components of a colorimeter are a light source, a cuvette containing the sample solution and a photocell for detecting the light passed through the solution.
The instrument is also equipped with either colored filters or specific LEDs to generate color. The output from a colorimeter may be displayed by an analog or digital meter in terms of transmittance or absorbance.
In addition, a colorimeter may contain a voltage regulator for protecting the instrument from fluctuations in mains voltage. Some colorimeters are portable and useful for on-site tests, while others are larger, bench-top instruments, which are useful for laboratory testing.
Working Principle
The colorimeter is based on Beer-Lambert's law, according to which the absorption of light transmitted through the medium is directly proportional to the medium concentration.
In a colorimeter, a beam of light with a specific wavelength is passed through a solution via a series of lenses, which navigate the colored light to the measuring device. This analyzes the color compared to an existing standard. A microprocessor then calculates the absorbance or percent transmittance. If the concentration of the solution is greater, more light will be absorbed, which can be identified by measuring the difference between the amount of light at its origin and that after passing the solution.
A colorimeter is a light-sensitive device used for measuring the transmittance and absorbance of light passing through a liquid sample. The device measures the intensity or concentration of the color that develops upon introducing a specific reagent into a solution.
There are two types of colorimeters — color densitometers, which measure the density of primary colors, and color photometers, which measure the color reflection and transmission.
Design of Colorimeter
The three main components of a colorimeter are a light source, a cuvette containing the sample solution and a photocell for detecting the light passed through the solution.
The instrument is also equipped with either colored filters or specific LEDs to generate color. The output from a colorimeter may be displayed by an analog or digital meter in terms of transmittance or absorbance.
In addition, a colorimeter may contain a voltage regulator for protecting the instrument from fluctuations in mains voltage. Some colorimeters are portable and useful for on-site tests, while others are larger, bench-top instruments, which are useful for laboratory testing.
Working Principle
The colorimeter is based on Beer-Lambert's law, according to which the absorption of light transmitted through the medium is directly proportional to the medium concentration.
In a colorimeter, a beam of light with a specific wavelength is passed through a solution via a series of lenses, which navigate the colored light to the measuring device. This analyzes the color compared to an existing standard. A microprocessor then calculates the absorbance or percent transmittance. If the concentration of the solution is greater, more light will be absorbed, which can be identified by measuring the difference between the amount of light at its origin and that after passing the solution.
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