Question

Which dna will run fast from supercoiled, linear dna and cirvcular?

Answer 1

When a pool of plasmid sample containing supercoiled, opencircular and linear form is run on agarose gel, supercoiled DNA will run faster which is the bottom most band, next is thenicked form i.e. opencircular form which is the middle band and the top most band in the gel is the linear or relaxed form (Fig. 4).