Question

Which enzyme is used to cut a gene out of a bacterial plasmid

Answer 1

Answer:

EcoRI or a similar restriction enzyme is used to cut the gene of interest from the organism. The same restriction enzyme is used to open a bacterial plasmid up for gene insertion. The gene of interest is inserted into the bacterial plasmid via an enzyme called DNA ligase.

Answer 2

Answer:

A restriction enzyme like EcoRl, EcoRll can be used. They are obtained from Escherichea coli