Which formula is use to calculate biological activity using ec50 value?
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It is very easy to calculate the EC50 value of any compounds by using both graph and the excel. Just evaluate the efficacy of the compound of interest in different concentration and plot the graph. Efficacy in Y axis and the concentration in X axis. then you can easily correlate the value of EC50 by using simple formula y=mx+c where y is 50 and m and c are the constants. x is the concentration you needed. Follow the following video in you tube for further clearance.http://www.youtube.com/watch?v=fMghQw2Ry2c
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