Question

Which gel electrophoresis should be used to separate DNA mixture having size difference of single nucleotide? * 1 point 5 % agarose gel electrophoresis 2 % agarose gel electrophoresis PAGE with TBE buffer PAGE with Tris-glycine buffer

Answer 1

Answer:

For a standard agarose gel electrophoresis, a 0.8% gel gives good separation or resolution of large 5–10kb DNA fragments, while 2% gel gives good resolution for small 0.2–1kb fragments. 1% gels is often used for a standard electrophoresis.

Explanation:

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Answer 2

Answer:

A laboratory technique called gel electrophoresis is used to divide combinations of DNA, RNA, or proteins based on their molecular sizes. In gel electrophoresis, the molecules that need to be separated are forced through a gel that has tiny pores by an electrical field.

Explanation:

• With the aid of various sizes-AgNPs standards, the effects of various stabilizing agents (PEG, SDS, and sodium dodecylbenzenesulfonate), buffers (TBE and Tris Glycine), and functionalizing agents (mercaptosuccinic acid (TMA) and proteins) have been examined.
• The varied sizes of AgNPs standards were caused by the use of 1% SDS, 0.1% TMA, and Tris Glycine in the gel, electrophoresis buffer, and loading buffer. These components moved in accordance with their size/charge ratio (obtaining a linear relationship between apparent mobility and mean diameter).
• The behavior of the AgNPs in the industrial product (which had a casein matrix) changed drastically after employing SDS and TMA, making it impossible to characterize their size.

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