which instrument is used for observing the temporary mount of cells? how should one observe the onion peel under it?
Answers
Answer:
Explanation:
we can use a microscope either compound or eiectronic.
how to observe
Add a drop of water at the center of the microscopic slide
Having pulled of a thin membrane from the onion layer, lay it at the center of the microscopic slide (the drop of water will help flatten the membrane)
Add a drop of iodine solution on the onion membrane (or methylene blue)
Gently lay a microscopic cover slip on the membrane and press it down gently using a needle to remove air bubbles.
Touch a blotting paper on one side of the slide to drain excess iodine/water solution,
Place the slide on the microscope stage under low power to observe.
Adjust focus for clarity to observe.
Answer:Pour some distilled water into a watch glass.
Peel off a leaf from half a piece of onion and using the forceps, pull out a piece of transparent onion peel (epidermis) from the leaf.
Put the epidermis in the watch glass containing distilled water.
Take a few drops of safranin solution in a dropper and transfer this into another watch glass.
Using a brush, transfer the peel into the watch glass containing the safranin solution.
Let this remain in the Safranin solution for 30 seconds, so that the peel is stained.
Take the peel from the Safranin solution using the brush and place it in the watch glass containing the distilled water.
Take a few drops of glycerine in a dropper and pour 2-3 drops at the center of a dry glass slide.
Using the brush, place the peel onto the slide containing glycerine.
Take a cover slip and place it gently on the peel with the aid of a needle.
Remove the extra glycerine using a piece of blotting paper.
Place this glass side on the stage of the compound microscope and view it.
Explanation: