Biology, asked by Varadnb8509, 1 year ago

which is the best method to purify his tagged protein

Answers

Answered by graxx

Hi there

Nickel, cobalt and copper. Nickel is the most widely available metal ion for purifying His-tagged proteins. Nickel generally provides good binding efficiency to His-tagged proteins but also tends to bind nonspecifically to endogenous proteins that contain histidine clusters.

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