Question

Which is the most widely used chemical for protoplast fusion as fusogen?

Answer 1

Explanation:

Sodium nitrate (NaN03), polyethylene glycol (PEG), Calcium ions (Ca2+), Polyvinyl alcohol etc. are the most commonly used protoplast fusion inducing agents which are commonly known as chemical fusogens.

Answer 2

Answer:

Polyethylene glycol (PEG) is used as the fusogen agent in protoplast fusion  A fusogen is any chemical that is required for fusing used in the fusing of two cells together.  for example polyvinyl alcohol and  Calcium ions (Ca2).

Explanation:

Protoplast inducing agents can be physical or chemical types. These agents can be used in protoplast fusion.

PEG is mostly used because of its high fusion efficiency, cheap cost, easy availabilty and ease of handling.  When PEG  is put on the cell it causes the molecules of the protoplast to  induce agglutination of protoplasts hence  cause the reversible breakdown of  membrane  and then fusion of the protoplasts.