Question

which is the principle of gram stain ?

Answer 1

Microbes with thicker cell walls retain the color.

Answer 2

Isse jayada meko nhi pata...

#Ram ram

The outer cell wall of Gram Negative Bacteria is made of lipoproteins ....

Bacteria are stained with Crystal Violet and Iodine solution is used as a mordant ...

After that they are washed with alcohol or Acetone solutions ...

Lipoprotiens present in the cell wall of Gram Negative bacteria are soluble in alcohol and acetone and the purple colour hence disappears...

Instead of cell wall of Gram Positive Bacteria is made up of peptidoglycan and thus takes the gram stain .....