Question

Which is used as vector for cloning genes into several dicot plants?

Answer 1

yeast is correct ans

Answer 2

Vectors for cloning genes in plants

• Tumour inducing plasmid (Ti) of soil bacterium.

• Agrobacterium tumefaciens has been made non virylent by deleting the part that is responsible for its virulence.

• However the modified plasmid does contain the tumour inducing gene T-DNA.

• It is however employed as recognition or cloning site.

• A foreign gene is inserted in T-DNA region of the plasmid.

• The T gene becomes inactive.

• The recombined or cloning vector is now passed into plasmid free bacterium that is allowed to infect the plant or single cells in tissue culture.

• The plasmid with the desired inserted gene passes into variuos parts.

• The inserted gene expresses its effect.

• Agrobacterium tumifaciens normally infects only dicot plants.

• The one with modified nonvirulent plasmid can also cause genetic changes in monocots.