which method is used in proximate analysis of protein, write the principle and procedure involved in the analysis.
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The proximate system for routine analysis of animal feedstuffs was devised in the mid-nineteenth century at the Weende Experiment Station in Germany (Henneberg and Stohmann, 1860, 1864). It was developed to provide a top level, very broad, classification of food components. The system consists of the analytical determinations of water (moisture), ash, crude fat (ether extract), crude protein and crude fibre. Nitrogen-free extract (NFE), more or less representing sugars and starches, is calculated by difference rather than measured by analysis.
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