which method is used in proximate analysis of protein, write the principle and procedure involved in the analysis.
Answers
Explanation:
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Answer:
proximate analysis is the analysis of macromolecules present in food.
Kjeldahl method is used in proximate analysis of protein .
Kjeldahl method was developed by kjeldahl
Explanation:
The protein is digested in a acidic environments so that it releases nitrogen which is determined by titration method.
.The amount of protein is then calculated from nitrogen concentration of the food.
The method is divided into 3 steps.
1.Digestion.
2. neutralisation.
3.titration.
1 Digestion-The food sample is weighed into a Digestion flask and then it is heated in the presence of sulfuric acid.anhydrous sodium
.sulfate and a catalyst such as mercury .
Digestion converts any nitrogen in the food into ammonia and other organic matter to co2 and H2o.Ammonia gas remain in the form of ammonium ion that binds to the sulfate.
2.neutralization-After Digestion the Digestion flask is connected to receiving flask with the help of tube .
.The solution in the Digestion flask is made alkaline by adding sodium hydroxide that converts the ammonium sulfate into ammonia gas.
.The ammonia gas moves out of the Digestion flask and into the receiving flask that contains boric acid .
.The pH of solution in receiving flask is low that converts the ammonia gas into ammonium ion and boric acid to borate ion.
3.titration-the nitrogen content is then estimated by titration of ammonium borate formed with sulfuric acid or hydrochloric acid.
.The concentration of hydrogen ion need to reach equivalent to the concentration of nitrogen.