Question

Which method transfection for making transgenic animals?

Answer 1

Explanation:

three principal methods used for the creation of transgenic animals are DNA microinjection, embryonic stem cell-mediated gene transfer and retrovirus-mediated gene transfer.

Answer 2

• Transgenic animals are genetically modified with the help of gene transfer methods like transfection.
• The desired gene from one organism is inserted into the DNA of the other organism with the help of vectors.
• As a result the subsequent generations also contain the desired gene or transgene.
• Transfection is the insertion of the desired gene into DNA/RNA by artificial methods.

Transfection can be done in 3 ways:

1. Transferring chromosome or fragments of chromosome in the cell.
2. Transferring the whole nuclei of the desired cell.
3. Transfer the nucleic acid of the desired cell into our cell.