Which method would you prefer to use when you have to separate different isoforms of a protein? sds-page 2-de 1-de bn-page?
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by performing 2D- gel electrophoresis: this step separates isoforms of proteins based on their molecular weight and isoelectric point (PI).
The PI is the ph at which a protein is neutral or has no net charge. So you can separate protein isoforms based on the differences on the net charge of each protein isoform.
The PI is the ph at which a protein is neutral or has no net charge. So you can separate protein isoforms based on the differences on the net charge of each protein isoform.
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