Which methodology is used while sequencing the total dna from a cell?
Answers
Answer:The DNA sequence is that the total DNA from a cell and is isolated, then converted into random fragments of relatively smaller size.
Explanation:
Answer:
the methadology of HGP(human genome project).
1)for sequencing the total DNA from cell is first isolated and broken down in relatively small size as fragments
2)these DNA fragments are clonned in suitable host using suitable vector,when bacteria is used as a vector they are called BAC(bacterial artificial chromosome)when yeast is used as a vector they are calld YAC(yeast artificial chromosome)
3)Frederick sanger developed a principle according to which the fragments of DNA are arranged accordingly .
4)for alignment of these sequence specialised computer based programmes were developed .
5)these sequences were annotated and were assigned to ach chromosome sequencing of chromosome 1 was completed only in may 2006 it was the last chromosome be sequenced
6)finally the genetic and physical map of genome were constructd by collecting information about certain repetative DNA sequences and DNA polymorphism based on endonuclease recognition site.
Explanation: