Question

Which methods of preservation of microbial culture includes the working stock?

Answer 1

Preserving Microbial Culture: Method # 4.

Preservation at Very Low Temperature:

The organisms are suspended in nutrient broth containing 15% glycerol. The suspension is frozen and stored at -15°C to -30°C. The availability of liquid nitrogen (temp -196°C) provides another main preserving stock culture. In this procedure culture are frozen with a protective agent (glycerol or dimethane sulphoxide) in sealed ampoules. The frozen culture are kept in liquid nitrogen refrigerator.