Which microscope uses the last dance invisible light for a observation
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Contents Home Courses Portland Community College Cascade Microbiology 2: How We See the Invisible World Expand/collapse global location
2.4: Staining Microscopic Specimens
Last updatedJun 15, 2019
2.3: Instruments of Microscopy
2.5: How We See the Invisible World (Exercises)
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SKILLS TO DEVELOP
Differentiate between simple and differential stains
Describe the unique features of commonly used stains
Explain the procedures and name clinical applications for Gram, endospore, acid-fast, negative capsule, and flagella staining
In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. We have already alluded to certain techniques involving stains and fluorescent dyes, and in this section we will discuss specific techniques for sample preparation in greater detail. Indeed, numerous methods have been developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope. Here, we will focus on the most clinically relevant techniques.