Question

Which of the following accurately describe the steps of recombinant DNA technology using bacteria?

1. plasmid & genes are cut --> cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria --> bacteria & rDNA plasmid multiply --> bacteria are broken open & protein is collected

2. cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria --> plasmid & genes are cut -->bacteria & rDNA plasmid multiply --> bacteria are broken open & protein is collected

3. plasmid & genes are cut -->bacteria & rDNA plasmid multiply --> bacteria are broken open & protein is collected-->cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria

4.cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria --> bacteria & rDNA plasmid multiply 

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Answer 1

Answer:

plasmid & genes are cut -->bacteria & rDNA plasmid multiply --> bacteria are broken open & protein is collected-->cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria

Answer 2

Answer:

Option 1 is the correct sequence.

Plasmid and gene of interest are cut using restriction enzymes and ligated using DNA ligase. This becomes a rDNA. This is inserted into a bacterial host cell. These host cells are grown and multiplied and the protein is collected from the bacteria.

Explanation:

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