Which of the following DNA is undigested by
restriction endonuclease in this diagram?
DNA
bands
B
с
Lane
Lane 1
Lane 2
Lane 3
Lane 4
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Answer:
Correct option is
B
(ii) and (iii)
The DNA fragments separate according to their size through the agarose gel, with smaller fragments moving farther away as compared to larger ones. The DNA fragments can be visualised by staining them with ethidium bromide follo
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The following DNA is not digested by the endonuclease block in this drawing is:
- The diagram is for gel electrophoresis where A is half cathode and B is half anode.
- C and E represent large amounts of undigested DNA and D stands for small strands of DNA.
- In genetic engineering tools, inhibitory enzymes detect palindromic DNA sequences and degrade them.
- The first endonuclease blocker was Hind II who used to cut DNA in certain areas.
- To date 900 enzymes have been divided into 230 species of bacteria.
- Restricted endonuclease are two types of exonuclease that separate DNA / RNA from the end and endonuclease cut DNA somewhere.
- Separation and isolation is a method of dividing the dynamic length of DNA by gel electrophoresis.
- The Cathode and anode represent both ends of the metal, the DNA placed in the cathode and when the electric field is produced the negatively charged DNA begins to move towards the end of the positive cathode.
- Larger pieces show slower movement while shorter pieces show faster movement.
- The DNA fragment is detected with the help of Lithium bromide, later released from the agarose gel.
- Then the merging of the vectors occurs by initiating a multiplication that requires an optional mark to convert.
- The mutation leads to the transfer of foreign DNA or plasmid that contains the passenger DNA into binding cells.
- DNA is later inserted into a given space.
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