Question

Which of the following enzyme is used to break the bacteria cell wall to release DNA and other bio micro molecules

Answer 1

Gross Morphology

Bacteria have characteristic shapes (cocci, rods, spirals, etc.) and often occur in characteristic aggregates (pairs, chains, tetrads, clusters, etc.). These traits are usually typical for a genus and are diagnostically useful.

Cell Structure

Prokaryotes have a nucleoid (nuclear body) rather than an enveloped nucleus and lack membrane-bound cytoplasmic organelles. The plasma membrane in prokaryotes performs many of the functions carried out by membranous organelles in eukaryotes. Multiplication is by binary fission.

Surface Structures

Flagella: The flagella of motile bacteria differ in structure from eukaryotic flagella. A basal body anchored in the plasma membrane and cell wall gives rise to a cylindrical protein filament. The flagellum moves by whirling about its long axis. The number and arrangement of flagella on the cell are diagnostically useful.

Pili (Fimbriae): Pili are slender, hairlike, proteinaceous appendages on the surface of many (particularly Gram-negative) bacteria. They are important in adhesion to host surfaces.

Capsules: Some bacteria form a thick outer capsule of high-molecular-weight, viscous polysaccharide gel; others have more amorphous slime layers. Capsules confer resistance to phagocytosis.

Important Chemical Components of Surface Structures

Cell Wall Peptidoglycans: Both Gram-positive and Gram-negative bacteria possess cell wall peptidoglycans, which confer the characteristic cell shape and provide the cell with mechanical protection. Peptidoglycans are unique to prokaryotic organisms and consist of a glycan backbone of muramic acid and glucosamine (both N-acetylated), and peptide chains highly cross-linked with bridges in Gram-positive bacteria (e.g., Staphylococcus aureus) or partially cross-linked in Gram-negative bacteria (e.g., Escherichia coli). The cross-linking transpeptidase enzymes are some of the targets for b-lactam antibiotics.

Teichoic Acids: Teichoic acids are polyol phosphate polymers bearing a strong negative charge. They are covalently linked to the peptidoglycan in some Gram-positive bacteria. They are strongly antigenic, but are generally absent in Gram-negative bacteria.

Lipoteichoic Acids: Lipoteichoic acids as membrane teichoic acids are polymers of amphiphitic glycophosphates with the lipophilic glycolipid and anchored in the cytoplasmic membrane. They are antigenic, cytotoxic and adhesins (e.g., Streptococcus pyogenes).

Lipopolysaccharides: One of the major components of the outer membrane of Gram-negative bacteria is lipopolysaccharide (endotoxin), a complex molecule consisting of a lipid A anchor, a polysaccharide core, and chains of carbohydrates. Sugars in the polysaccharide chains confer serologic specificity.