Which of the following enzymes does not require a co-factor?
A.
Hexokinase
B.
Aldolase
C.
Phosphofructokinase-1
D.
Pyruvate kinase
Answers
Answer:
B.
Aldolase
An inactive enzyme without the cofactor is called an apoenzyme, while the complete enzyme with cofactor is called a holoenzyme
Answer:
option c) Phosphofructokinase-1 does not have a co factor
Explanation:
option a)
The enzyme hexokinase requires a magnesium ion as a cofactor. Magnesium ions function to shield the negatively charged groups of ATP and facilitate the reaction with glucose. Coenzymes are also cofactors.
option b)
Aldolase is present in all animal and plant tissue, and in most microorganisms. ... Class II aldolase, found only in prokaryotes and lower eukaryotes, requires a bivalent metal cofactor (Heron and Caprioli 1975, London 1974, and Lebherz et al. 1973, Gefflaut et al. 1995).
option d)
Therefore, Mg2+ was concluded to be an important cofactor in the catalysis of PEP into pyruvate by pyruvate kinase. ... The binding of metal ions to the metal binding sites on pyruvate kinase enhances the rate of this reaction. The reaction catalyzed by pyruvate kinase is the final step of glycolysis
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