Question

Which of the following enzymes separates the two strands of DNA during replication?​

Answer 1

Answer:

. Helicase separates the two strands of DNA at the Replication Fork behind the topoisomerase. The enzyme responsible for catalyzing the addition of nucleotide substrates to DNA in the 5′ to 3′ direction during DNA replication.

Answer 2

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Helicase

Helicase separates the two strands of DNA at the Replication Fork behind the topoisomerase. The enzyme responsible for catalyzing the addition of nucleotide substrates to DNA in the 5′ to 3′ direction during DNA replication.

• DNA polymerase adds deoxyribonucleotides to the to the 3'-OH end of the growing polynucleotide chain.

• Helicase helps in unwinding the two strands of DNA double helix during replication.

• Gyrase helps in relaxing the tension or strain formed due to unwinding of DNA.

• Topoisomerase helps in relieving the tension created during unwinding of DNA.

Hence, the enzyme that catalyses the separation of DNA strands during DNA replication is Helicase.

So, the correct answer is 'Helicase'.