Question

Which of the following is correct w.r.t. PCR?

1. Denaturation at 48°C, separating the two DNA strands

2. Extension of primers at 98°C

3. Extension in the presence of heat stable DNA polymerase

4. Annealing with two sets of primers at 72°C

Answer 1

Answer:

ANSWER IS 3

Explanation:

FIRST STEP - DENATURATION AT 94 *C

SECOND STEP - ANNEALING/COOLING AT 54 *C

THIRD STEP - EXTENSION AT 72 *C IN PRESENCE OF TAQ POLYMEASE [ HEAT STABLE DNA POLYMERASE]

THANK YOU...