Question

Which of the following is indispensable in genetic engineering?
1. DNA ligase
2. Restriction endonuclease
3. RNA polymerase
4.Reverse transcriptase

Answer 1

Answer:

Restriction endonuclease is indispensable in genetic engineering.

The correct answer is 2. Restriction endonuclease.

Explanation:

Restriction enzymes also known as Restriction endonuclease are crucial tools in genomic research because they cut DNA at a precise place, allowing foreign DNA to be inserted for gene editing. Natural restriction enzymes aren't the only ones who can do it; artificial restriction enzymes, or AREs, have gained popularity in recent years. CRISPR-Cas9, a bacterial immune system that allows "cut-and-paste" gene editing, and TALENs, which are modified restriction enzymes, are two popular examples.

Restriction enzymes are generally divided into four types, based on subunit structure, cleavage conditions, sequence specificity and cofactor requirements.

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Answer 2

1. Restriction enzymes, also known as restriction endonucleases, are important tools in genomic research because they cut DNA at specific locations, allowing foreign DNA to be inserted for gene editing.
2. Natural restriction enzymes aren't the only ones capable of doing so; artificial restriction enzymes, or AREs, have grown in popularity in recent years.
3. Two popular examples are CRISPR-Cas9, a bacterial immune system that allows "cut-and-paste" gene editing, and TALENs, which are modified restriction enzymes.
4. In genetic engineering, restriction endonuclease is indispensable.

Hence, option B is correct.

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