Question

Which of the following is not an essential components of yac vector

Answer 1

Answer:

Yeast artificial chromosomes are genetically engineered chromosomes derived from the DNA of the yeast, Saccharomyces, which is then ligated into a bacterial plasmid.

By inserting large fragments of DNA, from 100–1000 kb, the inserted sequences can be cloned and physically mapped using a process called chromosome walking.

The primary components of a YAC are the ARS, centromere, and telomeres from Saccharomyces.

Additionally, selectable marker genes, such as antibiotic resistance and a visible marker, are utilized to select transformed yeast cells.

Without these sequences, the chromosome will not be stable during extracellular replication, and would not be distinguishable from colonies without the vector

Answer 2

The number one additives of a YAC are the ARS, centromere, and telomeres from S. cerevisiae. Additionally, selectable marker genes, inclusive of antibiotic resistance and a seen marker, are applied to pick out converted yeast cells.

Explanation:

• Yeast artificial chromosomes are genetically engineered chromosomes derived from the DNA of the yeast, Saccharomyces, that are then ligated right into a bacterial plasmid.
• By placing huge fragments of DNA, from 100–one thousand kb, the inserted sequences may be cloned and bodily mapped by the use of a manner known as chromosome walking.
• Without those sequences, the chromosome will now no longer be solid in the course of extracellular replication, and might now no longer be distinguishable from colonies without the vector.

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